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Feasibility study on replacing fish meal with S-peptide

1. Experimental design

Select 480 healthy juvenile South American white shrimp, with an initial weight of 0.5g, and treat each feed with 3 replicates, with 40 shrimp per replicate. The feed treatment for young shrimp includes a control group (fish meal group) and three experimental groups (S-peptide, S-peptide (sterilized), and fermented soybean meal D replacing part of the fish meal group, respectively). The feed formula is shown in Table 1. The experiment was conducted with full feeding, administered 4 times a day for 8 weeks. The experimental environment is 27.0 ± 1 ℃, equipped with a seawater circulation device (salinity 33ppt).

Table 1. Composition of experimental feed

2. Experimental results

2.1 Effect of Su Yi Peptide on the Growth Performance of Juvenile Shrimp

Whether sterilized or not, Su Yi Peptide successfully replaced 9 fish meal points. Among them, the non sterilized Su Yi Peptide group significantly increased the weight gain and specific growth rate of South American white shrimp compared to other groups (P<0.05), and the sterilized Su Yi Peptide still improved the production performance of South American white shrimp to a certain extent. The results indicate that the improvement of production performance of South American white shrimp by Su Yi peptide is attributed to probiotics and product antigen degradation.

2.2 Effects of Su Yi Peptide on Non specific Immunity of Juvenile Shrimp

There was no significant difference in the inhibition rate of superoxide dismutase among the groups (P>0.05), but the lysozyme activity of juvenile shrimp in the Su Yi Peptide group was significantly higher than that in the fish meal and fermented soybean meal D group (P<0.05). The lysozyme activity of the Su Yi Peptide (sterilized) group was significantly higher than that of the fermented soybean meal D group, and also improved to a certain extent compared to the fish meal group (Figure 6).

Application of Su Yi Peptide in Improving the Survival Rate and Water Quality of South American White Shrimp

1. Experimental design

Select 120 healthy South American white shrimp with an initial weight of 4.08+0.12g, with 3 replicates per group and 10 juvenile shrimps per replicate. The feed treatment consisted of a control group, a control&probiotic group (control group (+)), a fish meal replacement group (S-peptide group), and a fish meal replacement&probiotic group (S-peptide (+)), with probiotics sourced from a commercial brand B (Table 2). The experiment adopts full feeding, feeding 4 times a day for a period of 4 weeks. The experimental environment is 27.0 ± 2 ℃, equipped with a seawater circulation device (salinity 33ppt).

Table 2. Experimental Design

'X' indicates not added; 'O' means add

2. Experimental results

2.1 The effect of S-peptide on the survival rate of juvenile shrimp

In the fourth week of the experiment, the change of experimental water was stopped, and the mortality rate of each group of South American white shrimp increased. Among them, the control group had the highest mortality rate, reaching 50%, which was significantly higher than the other three groups (P<0.05). However, there was no significant difference in survival rate among the S-peptide group, S-peptide (+) group, and control group (+) (P>0.05). The survival rate of the S-peptide group was higher than 60%, slightly higher than that of the control group (+) (Figure 5).

2.2 Water regulating effect of Su Yi Peptide

After one week, the NO2 concentration in the water of the Rapid Peptide group and Rapid Peptide (+) group was significantly lower than that of the control group (P<0.05). At the same time, the Bacillus subtilis in the Rapid Peptide group had better control over the NO2 concentration in the water than the commercial brand B used in this experiment. In the second and fourth weeks of the experiment, the number of Bacillus subtilis per milliliter of water in the Su Yi Peptide group and Su Yi Peptide (+) group was significantly higher than that in the control group (P<0.05).

Conclusion

1. The improvement of production performance of South American white shrimp by Su Yi Peptide is attributed to various factors such as probiotics and antigen degradation in the product.

2. Suyipeptide improves the water quality and environment of South American white shrimp farming, while enhancing immunity and increasing the survival rate of South American white shrimp.